Molecular characterization of azoreductase and its potential for the decolorization of Remazol Red R and Acid Blue 29
- Authors
- Mustafa, Ghulam; Zahid, Muhammad Tariq; Bharat Kurade, Mayur; Mahadeo Patil, Swapnil; Shakoori, Farah Rauf; Shafiq, Zeeshan; Ihsan, Sidra; Ahn, Yongtae; Khan, Azmat Ali; Jeon, Byong-hun; Gacem, Amel
- Issue Date
- Oct-2023
- Publisher
- Elsevier Ltd
- Keywords
- Decolorization; Remazol red; Azoreductase; Biodegradation; Bioremediation
- Citation
- ENVIRONMENTAL POLLUTION, v.335, pp.1 - 11
- Indexed
- SCIE
SCOPUS
- Journal Title
- ENVIRONMENTAL POLLUTION
- Volume
- 335
- Start Page
- 1
- End Page
- 11
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/192970
- DOI
- 10.1016/j.envpol.2023.122253
- ISSN
- 0269-7491
- Abstract
- Azoreductase is a reductive enzyme that efficiently biotransformed textile azo dyes. This study demonstrated the heterologous overexpression of the azoreductase gene in Escherichia coli for the effective degradation of Remazol Red-R and Acid-Blue 29 dyes. The AzK gene of Klebsiella pneumoniae encoding a ≈22 kDa azoreductase enzyme was cloned into the pET21+C expression vector. The inoculum size of 1.5%, IPTG concentration of 0.5 mM, and incubation time of 6 h were optimized by response surface methodology a statistical tool. The crude extract showed 76% and 74%, while the purified enzyme achieved 94% and 93% decolorization of RRR and AB-29, respectively in 0.3 h. The reaction kinetics showed that RRR had a Km and Vmax value of 0.058 mM and 1416 U mg−1, respectively at an NADH concentration of 10 mM. HPLC and GC-MS analyses showed that RRR was effectively bio-transformed by azoreductase to 2-[3-(hydroxy-amino) benzene-1-sulfonyl and AB-29 to aniline and 3-nitrosoaniline. This study explored the potential of recombinant azoreductase isolated from K. pneumoniae in the degradation of toxic textile azo dyes into less toxic metabolites.
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