Sensitive fluorescent imaging of Salmonella enteritidis and Salmonella typhimurium using a polyvalent directed peptide polymer
- Authors
- Lee, Sang-Choon; Kim, Min-Seo; Yoo, Ki-Chun; Ha, Na-Reum; Moon, Ji-Young; Lee, Su-Jae; Yoon, Moon-Young
- Issue Date
- Aug-2017
- Publisher
- SPRINGER WIEN
- Keywords
- Food poisoning; Cell-phage display; Polyvalant directed peptide polymer; Fluorescein isothiocyanate; Succinimidyl 3-(2-pyridyldithio)propionate; RT-PCR
- Citation
- MICROCHIMICA ACTA, v.184, no.8, pp.2611 - 2620
- Indexed
- SCIE
SCOPUS
- Journal Title
- MICROCHIMICA ACTA
- Volume
- 184
- Number
- 8
- Start Page
- 2611
- End Page
- 2620
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/19498
- DOI
- 10.1007/s00604-017-2240-1
- ISSN
- 0026-3672
- Abstract
- The authors describe three fluorescein-conjugated peptides generated by cell-phage display for use as a diagnostic probes for fluorescent detection and imaging of Salmonella enteritidis and Salmonella typhimurium. The authors also designed a polyvalent-directed peptide polymer synthesized with poly-D-lysine and bifunctional succinimidyl 3-(2pyridyldithio) propionate with an affinity and sensitivity that is higher by more than an order of magnitude compared to single peptides due to multiple binding site interactions. In order to establish a diagnostic system for food poisoning, imaging analysis was performed using fluorescence microscopy. The limit of detection of the diagnostic system based on polyvalent directed peptide interaction is 10(2) colony-forming units per mL for Salmonella.
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