Competent antigen-presenting cells are generated from the long-term culture of splenocytes with granulocyte-macrophage colony-stimulating factor
- Authors
- Ryu, Seul Hye; Na, Hye Young; Sohn, Moah; Choi, Wanho; In, Hyunju; Shin, Hyun Soo; Choi, Jae-Hoon; Park, Chae Gyu
- Issue Date
- Aug-2017
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Antigen-presenting cells; Dendritic cells; GM-CSF; Hematopoiesis; Spleen
- Citation
- IMMUNOLOGY LETTERS, v.188, pp.96 - 107
- Indexed
- SCIE
SCOPUS
- Journal Title
- IMMUNOLOGY LETTERS
- Volume
- 188
- Start Page
- 96
- End Page
- 107
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/19500
- DOI
- 10.1016/j.imlet.2017.06.010
- ISSN
- 0165-2478
- Abstract
- Dendritic cells (DCs) are routinely produced from the culture of mouse bone marrow (BM) with granulocyte macrophage colony-stimulating factor (GM-CSF) within a period of 10 days. Although splenic extramedullary myelopoiesis was suggested to occur under the influence of GM-CSF, the hematopoietic outcome of splenic culture with GM-CSF has not been scrutinized. We have cultured mouse splenocytes with GM-CSF for an extended period of time, where we discovered that the CD11b(+)CD11c(+) cells began to proliferate prominently after 10 days and their number increased until the 4th week of the culture. In parallel experiments, FMS-like tyrosine kinase 3 (FLT3) and its ligand, FLT3L, were not found to influence the culture of splenocytes. Like DCs in the culture of BM with GM-CSF, a distinct population of CD11b(+)CD11c(+)MHC IIhi cells was readily identified as DCs in the long-term culture of splenocytes. After being isolated and plated overnight the CD11b(+)CD11c(+)MHC Il(hi) cells exhibited non-adherent dendritic morphology, while the other CD11b(+)CD11c(+) cells became adherent. Besides, these CD11b(+)CD11c(+)MHC IIhi cells possessed relatively weak endocytic and phagocytic abilities but displayed strong antigen-presenting capacities, revealing DC-like characteristics; in contrast, the other CD11b(+)CD11c(+) cells showed strong endocytosis and phagocytosis of antigens but were poor at antigen presentation, indicating macrophage-like traits. Therefore, we demonstrated that phenotypically as well as functionally genuine DCs are generated in the long-term culture of splenocytes with GM-CSF.
- Files in This Item
-
Go to Link
- Appears in
Collections - 서울 자연과학대학 > 서울 생명과학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/19500)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.