Graying the self-assembly of gold nanoparticles for improved enzyme activity assays
- Authors
- Kim, Gae Baik; Lee, Jin Oh; Kim, Young-Pil
- Issue Date
- Jul-2017
- Publisher
- ELSEVIER SCIENCE SA
- Keywords
- Gold nanoparticle; Silver enhancement; Signal amplification; Self-assembly; Enzyme activity
- Citation
- SENSORS AND ACTUATORS B-CHEMICAL, v.246, pp.271 - 277
- Indexed
- SCIE
SCOPUS
- Journal Title
- SENSORS AND ACTUATORS B-CHEMICAL
- Volume
- 246
- Start Page
- 271
- End Page
- 277
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/19562
- DOI
- 10.1016/j.snb.2017.02.067
- ISSN
- 0925-4005
- Abstract
- Despite the simplicity and usability of gold nanoparticle (AuNP)-based colorimetry in a large panel of bioassays, this technique still suffers from a poor detection limit. To overcome this issue, we report an improved AuNP-based colorimetric assay in combination with simple centrifugation and silver enhancement. As a proof-of-concept, two types of enzymes (i.e., protein phosphatase and protease) were constructed based on the self-assembly of thiol-stabilized AuNPs in the presence of peptides and metal ions. When the AuNP solutions were subjected to a short cycle of centrifugation and silver enhancement, the signal-to-background ratio (SBR) was distinctly improved by a factor of 5–10 in both enzyme activity assays, as compared to that of AuNP-based colorimetry. The detection limit achieved by using the silver enhancement was determined to be improved by a factor of 1.0–3.4. Furthermore, grayscale images of the silver enhancement allowed for a rapid and simple enzyme assay that did not require measuring the absorbance. Due to its ability to improve the detection senstivity in a facile way, we anticipate that this approach will be suitable for use in many AuNP colorimetric assays.
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