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Exploring the dynamic nature of divalent metal ions involved in DNA cleavage by CRISPR-Cas12aopen access
- Authors
- Son, Heyjin; Park, Jaeil; Choi, You Hee; Jung, Youngri; Lee, Joong-Wook; Bae, Sangsu; Lee, Sanghwa
- Issue Date
- Feb-2022
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- CHEMICAL COMMUNICATIONS, v.58, no.12, pp 1978 - 1981
- Pages
- 4
- Indexed
- SCIE
SCOPUS
- Journal Title
- CHEMICAL COMMUNICATIONS
- Volume
- 58
- Number
- 12
- Start Page
- 1978
- End Page
- 1981
- ISSN
- 1359-7345
1364-548X
- Abstract
- CRISPR-Cas12a has been widely used in genome editing and nucleic acid detection. In both of these applications, Cas12a cleaves target DNA in a divalent metal ion-dependent manner. However, when and how metal ions contribute to the cleavage reaction is unclear. Here, using a single-molecule FRET assay, we reveal that these metal ions are necessary for stabilising cleavage-competent conformations and that they are easily exchangeable, suggesting that they are dynamically coordinated.
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Collections - 서울 자연과학대학 > 서울 화학과 > 1. Journal Articles
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