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Complete Genome Sequence of the Poly(Butylene Adipate-co-Terephthalate) (PBAT)-Degrading Piscinibacter sp. SBD11-9, Isolated from an Agricultural Soil with Long-Term Use of Biodegradable Mulch Filmsopen access

Authors
Chung, Joon-HuiYeon, JehyeongKo, Young-joonKim, DayeonAn, SihyunWeon, Hang-yeonJeon, YunaYoon, Gyeong SeokSang, Byoung InAhn, Jae-hyung
Issue Date
Dec-2025
Publisher
한국미생물·생명공학회
Keywords
Piscinibacter; PBAT-degrading bacteria; genome
Citation
Microbiology and Biotechnology Letters, v.53, no.4, pp 679 - 682
Pages
4
Indexed
SCOPUS
KCI
Journal Title
Microbiology and Biotechnology Letters
Volume
53
Number
4
Start Page
679
End Page
682
URI
https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/210367
DOI
10.48022/mbl.2511.11001
ISSN
1598-642X
2234-7305
Abstract
The poly(butylene adipate-co-terephthalate) (PBAT)-degrading Piscinibacter sp. SBD11-9 was isolated from a maize field soil in Yeongwol-gun, Gangwon-do, Republic of Korea, where biodegradable mulch films were applied over 8 years. Whole genome analysis revealed that the genome of strain SBD11-9 (5.386 Mb) comprises one chromosome (5,335,603 bp) and one plasmid (50,699 bp), with G+C contents of 67.6%, and 63.7%, respectively. It also suggested that the strain is new species of the genus Piscinibacter. Strain SBD11-9 possesses five genes encoding potential poly(ethylene terephthalate) hydrolases (PETases), one of which showed a high protein identity (97.2%) to a PETase reported to degrade PBAT. This genome provides the clues for the biodegradation of PBAT-based mulch films.
PBAT를 분해하는 Piscinibacter sp. SBD11-9는 생분해성 멀칭필름을 8년 이상 사용한 대한민국 강원도 영월군의 옥수수 재배 포장에서 분리되었다. 유전체 분석결과, SBD11-9의 유전체는 염색체 1개(5,335,603 bp)와 플라스미드 1개(50,699 bp)로 이루어져 있으며, 각각의 G+C 함량은 67.6%, 63.7%이었다. 유전체 분석 결과 SBD11-9 균주는 Piscinibacter 속의 신종으로 동정되었다. SBD11-9 균주는 PETase를 암호화하는 다섯 개의 유전자를 보유하고 있으며 이 중 1개는 기존 연구에서 PBAT를 분해한다고 알려진 PETase와 높은 단백질 상동성(97.2%)을 보였다. 이 유전체 정보는 PBAT 기반 멀칭필름의 생분해와 관련된 단서를 제공한다.
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COLLEGE OF ENGINEERING (DEPARTMENT OF CHEMICAL ENGINEERING)
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