CpG Oligodeoxynucleotide Inhibits Cockroach-Induced Asthma via Induction of IFN-γ+ Th1 Cells or Foxp3+ Regulatory T Cells in the Lungopen access
- Authors
- Kim, Do-Hyun; Sohn, Jung-Ho; Park, Hong-Jai; Lee, Jae-Hyun; Park, Jung-Won; Choi, Je-Min
- Issue Date
- May-2016
- Publisher
- KOREAN ACAD ASTHMA ALLERGY & CLINICAL IMMUNOLOGY
- Keywords
- CpG-ODN; cockroach allergen; Th2 inflammation; immune modulation
- Citation
- ALLERGY ASTHMA & IMMUNOLOGY RESEARCH, v.8, no.3, pp.264 - 275
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- ALLERGY ASTHMA & IMMUNOLOGY RESEARCH
- Volume
- 8
- Number
- 3
- Start Page
- 264
- End Page
- 275
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/23102
- DOI
- 10.4168/aair.2016.8.3.264
- ISSN
- 2092-7355
- Abstract
- Purpose: CpG oligodeoxynucleotide (CpG-ODN), a TLR9 agonist, activates innate immunity and induces Th1 response. Although the immune modulatory effect of CpG-ODN has been extensively studied, its function in cockroach extract-induced allergic asthma has not been studied. Here, we investigated the inhibitory function of CpG-ODN in cockroach extract-induced asthma in mice with different treatment schemes. Methods: Scheme 1: BALB/C mice were intra-nasally co-administered by cockroach extract and CpG-ODN twice a week for 3 weeks; Scheme 2: The mice were intra-nasally pre-treated with CpG-ODN at day 0 and cockroach allergen challenge was performed from day 3 as in scheme 1. Scheme 3: Cockroach allergen challenge was performed as in scheme 1 and CpG-ODN was post-treated at day 21. Then, BAL cell count, flow cytometric analysis of alveolar macrophages, regulatory T cells, and lung tissue histology, Th1 and Th2 cytokines, serum IgE, cockroach specific IgE, IgG1/IgG2a ratio, and airway hyper-responsiveness were evaluated. Results: Mice with repeated intra-nasal exposure to CpG-ODN showed a dramatic decrease in eosinophilic inflammation, goblet cell hyperplasia, and airway hyper-responsiveness with reduction of IL-13, IL-5, and serum IgE, cockroach specific IgE and IgG1/IgG2a ratio. This inhibitory function might be related to the up-regulation of IL-10 and CD4(+)Foxp3(+) regulatory T cells in the lung. Interestingly, one-time challenge of CpG-ODN either prior or posterior to cockroach extract exposure could modulate airway inflammation and hyper-responsiveness via increase of Th1 response. Conclusions: Collectively, our data suggest that CpG-ODN treatment modulates Th2 inflammation in the lung by induction of regulatory T cells or Th1 response in a cockroach-induced asthma model.
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