Heat Shock Protein 90 Inhibitor Decreases Collagen Synthesis of Keloid Fibroblasts and Attenuates the Extracellular Matrix on the Keloid Spheroid Model
- Authors
- Lee, Won Jai; Lee, Ju Hee; Ahn, Hyo Min; Song, Seung Yong; Kim, Yong Oock; Lew, Dae Hyun; Yun, Chae-Ok
- Issue Date
- Sep-2015
- Publisher
- LIPPINCOTT WILLIAMS & WILKINS
- Citation
- PLASTIC AND RECONSTRUCTIVE SURGERY, v.136, no.3, pp.328e - 337e
- Indexed
- SCIE
SCOPUS
- Journal Title
- PLASTIC AND RECONSTRUCTIVE SURGERY
- Volume
- 136
- Number
- 3
- Start Page
- 328e
- End Page
- 337e
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/24877
- DOI
- 10.1097/PRS.0000000000001538
- ISSN
- 0032-1052
- Abstract
- Background: The 90-kDa heat-shock protein (heat-shock protein 90) is an abundant cytosolic chaperone, and inhibition of heat-shock protein 90 by 17-allylamino-17-demethoxygeldanamycin (17-AAG) compromises transforming growth factor (TGF)--mediated transcriptional responses by enhancing TGF- receptor I and II degradation, thus preventing Smad2/3 activation. In this study, the authors evaluated whether heat-shock protein 90 regulates TGF- signaling in the pathogenesis and treatment of keloids.
Methods: Keloid fibroblasts were treated with 17-AAG (10 M), and mRNA levels of collagen types I and III were determined by real-time reverse- transcriptase polymerase chain reaction. Also, secreted TGF-1 was assessed by enzyme-linked immunosorbent assay. The effect of 17-AAG on protein levels of Smad2/3 complex was determined by Western blot analysis. In addition, in 17-AAG-treated keloid spheroids, the collagen deposition and expression of major extracellular matrix proteins were investigated by means of Masson trichrome staining and immunohistochemistry.
Results: The authors found that heat-shock protein 90 is overexpressed in human keloid tissue compared with adjacent normal tissue, and 17-AAG decreased mRNA levels of type I collagen, secreted TGF-ss 1, and Smad2/3 complex protein expression in keloid fibroblasts. Masson trichrome staining revealed that collagen deposition was decreased in 17-AAG-treated keloid spheroids, and immunohistochemical analysis showed that expression of collagen types I and III, elastin, and fibronectin was markedly decreased in 17-AAG-treated keloid spheroids.
Conclusion: These results suggest that the antifibrotic action of heat-shock protein 90 inhibitors such as 17-AAG may have therapeutic effects on keloids.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 서울 공과대학 > 서울 생명공학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/24877)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.