A novel approach for amine derivatization of MoS2 nanosheets and their application toward label-free immunosensor
- Authors
- Kukkar, Manil; Tuteja, Satish K.; Kumar, Parveen; Kim, Ki-Hyun; Bhadwal, Akhshay Singh; Deep, Akash
- Issue Date
- Aug-2018
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- MoS2; Electrochemical derivatization; Amine functionality; EDC-NHS; Prostate-specific antigen; Immunosensor
- Citation
- ANALYTICAL BIOCHEMISTRY, v.555, pp.1 - 8
- Indexed
- SCIE
SCOPUS
- Journal Title
- ANALYTICAL BIOCHEMISTRY
- Volume
- 555
- Start Page
- 1
- End Page
- 8
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/3069
- DOI
- 10.1016/j.ab.2018.05.029
- ISSN
- 0003-2697
- Abstract
- The application of molybdenum disulfide (MoS2) nanosheets has assumed great significance in the design of next-generation biosensors. The immobilization of biomolecules on MoS2 nanosheets has generally been achieved via hydrophobic interactions or through other complicated surface modifications. In this work, we report a novel strategy for electrochemically assisted amine derivatization of MoS2 nanosheets. This newly proposed approach helped to immobilize the MoS2 nanosheets with antibodies via facile EDC/NHS {N-(3-dimethylaminopropy1)-N-(ethylcarbodiimide/N-hydroxysuccinimide)} cross-linking chemistry. The MoS2 nanosheets were first exfoliated and then electrochemically modified with 2-aminobenzylamine. Through a subsequent bioconjugation of the above amine-derivatized MoS2 nanosheets with anti-prostate-specific antigen (PSA) antibodies, an inununosensing device was realized for the detection of the 'prostate specific antigen'. The application of the proposed immunosensor was characterized with a low detection limit (10(-3) ng/mL) over a very wide quantitation range (10(-3) to 200 ng/mL).
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