MOF-Bacteriophage Biosensor for Highly Sensitive and Specific Detection of Staphylococcus aureus
- Authors
- Bhardwaj, Neha; Bhardwaj, Sanjeev K.; Mehta, Jyotsana; Kim, Ki-Hyun; Deep, Akash
- Issue Date
- Oct-2017
- Publisher
- AMER CHEMICAL SOC
- Keywords
- Staphylococcus aureus; bacteriophages; MOF; fluorescence; detection
- Citation
- ACS APPLIED MATERIALS & INTERFACES, v.9, no.39, pp.33589 - 33598
- Indexed
- SCIE
SCOPUS
- Journal Title
- ACS APPLIED MATERIALS & INTERFACES
- Volume
- 9
- Number
- 39
- Start Page
- 33589
- End Page
- 33598
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/4043
- DOI
- 10.1021/acsami.7b07818
- ISSN
- 1944-8244
- Abstract
- To produce a sensitive and specific biosensor for Staphylococcus aureus, bacteriophages have been interfaced with a water-dispersible and environmentally stable metal-organic framework (MOF), NH2-MIL-53(Fe). The conjugation of the MOF with bacteriophages has been achieved through the use of glutaraldehyde as cross-linker. Highly sensitive detection of S. aureus in both synthetic and real samples was realized by the proposed MOF-bacteriophage' biosensor based on the photoluminescence quenching phenomena; limit of detection (31 CFU/mL) and range of detection (40 to 4 X 10(8) CFU/mL). This is the first report exploiting the use of an MOF-bacteriophage complex for the biosensing of S. aureus. The results of our study highlight that-the proposed biosensor is more sensitive than most of the previous methods while exhibiting some advanced features like specificity) regenerability, extended range of linear 'detection, and stability for long-term storage (even at room temperature).
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