Effect of Relaxin Expression from an Alginate Gel-Encapsulated Adenovirus on Scar Remodeling in a Pig Modelopen access
- Authors
- Yun, In Sik; Kang, Eunhye; Ahn, Hyo Min; Kim, Yong Oock; Rah, Dong Kyun; Roh, Tai Suk; Lee, Won Jai; Yun, Chae-Ok
- Issue Date
- Sep-2019
- Publisher
- YONSEI UNIV COLL MEDICINE
- Keywords
- Gene therapy; scar remodeling; relaxin; adenovirus; alginate gel; pig scar model
- Citation
- YONSEI MEDICAL JOURNAL, v.60, no.9, pp.854 - 863
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- YONSEI MEDICAL JOURNAL
- Volume
- 60
- Number
- 9
- Start Page
- 854
- End Page
- 863
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/4540
- DOI
- 10.3349/ymj.2019.60.9.854
- ISSN
- 0513-5796
- Abstract
- Purpose: Relaxin (RLX) is a transforming growth factor-beta 1 (TGF-beta 1) antagonist that is believed to function as a potent collagen re-arranger and a major suppressor of extracellular matrix components. Adenoviruses (Ads) are accepted vectors for cancer gene therapy. However, repeated treatments of Ad are limited by short-term biological activity in vivo. The efficacy of sustained RLX expression to scar remodeling was assessed using an injectable alginate gel-matrix system. Materials and Methods: Pig scar tissue was treated with relaxin-expressing Ad loaded in alginate gel (gel/Ad-RLX). Surface areas, color, and pliability of scars were compared, and various factors influencing scar formation and collagen arrangement were analyzed. Results: Gel/Ad-RLX decreased scar size, color index, and pliability. Immunohistochemistry showed decreased levels of major extracellular matrix proteins in the gel/Ad-RLX-treated group. Furthermore, treatment with gel/Ad-RLX reduced expression of tissue inhibitor of metalloproteinase-1 and alpha-smooth muscle actin and markedly increased expression of matrix metalloproteinase-1 in pig scar tissues. Gel/Ad-RLX also significantly downregulated TGF-beta 1 and upregulated TGF-beta 3 mRNAs in pig scar tissues. Conclusion: These results support a prominent role for RLX in scar remodeling and suggest that gel/Ad-RLX may have therapeutic effects on scar formation.
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