Hydrogels with an embossed surface: An all-in-one platform for mass production and culture of human adipose-derived stem cell spheroids
- Authors
- Kim, Se-jeong; Park, Jaesung; Byun, Hayeon; Park, Young-Woo; Major, Luke G.; Lee, Dong Yun; Choi, Yu Suk; Shin, Heungsoo
- Issue Date
- Jan-2019
- Publisher
- ELSEVIER SCI LTD
- Keywords
- 3D cell culture; Stem cell spheroid; 3D printing; Surface structure; Hydrogel
- Citation
- BIOMATERIALS, v.188, pp.198 - 212
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOMATERIALS
- Volume
- 188
- Start Page
- 198
- End Page
- 212
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/4628
- DOI
- 10.1016/j.biomaterials.2018.10.025
- ISSN
- 0142-9612
- Abstract
- Stem cell spheroids have been studied extensively in organoid culture and therapeutic transplantation. Herein, hydrogels with an embossed surface (HES) were developed as an all-in-one platform that can enable the rapid formation and culture of a large quantity of size-controllable stem cell spheroids. The embossed structure on the hydrogel was adjustable according to the grit designation of the sandpaper. Human adipose-derived stem cells (hADSCs) were rapidly assembled into spheroids on the hydrogel, with their size distribution precisely controlled from 95 +/- 6 pm to 181 +/- 15 mu m depending on surface roughness. The hADSC spheroids prepared from the HES demonstrated expression of sternness markers and differentiation capacity. In addition, HES-based spheroids showed significantly greater VEGF secretion than spheroids grown on a commercially available low-attachment culture plate. Exploiting those advantages, the HES-based spheroids were used for 3D bioprinting, and the spheroids within the 3D-printed construct showed improved retention and VEGF secretion compared to the same 3D structure containing single cell suspension. Collectively, HES would offer a useful platform for mass fabrication and culture of stem cell spheroids with controlled sizes for a variety of biomedical applications.
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