Agglomeration of human dermal fibroblasts with ECM mimicking nano-fragments and their effects on proliferation and cell/ECM interactions
- Authors
- Ahmad, Taufiq; Shin, Young Min; Lee, Jinkyu; Shin, Hyeok Jun; Perikamana, Sajeesh Kumar Madhurakart; Shin, Heungsoo
- Issue Date
- Nov-2018
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- Nano-fragment (NF); NF-spheroids; Hybrid spheroids; Composite spheroid; 3D culture; Polydopamine; ECM
- Citation
- JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, v.67, pp.80 - 91
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
- Volume
- 67
- Start Page
- 80
- End Page
- 91
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/4661
- DOI
- 10.1016/j.jiec.2018.06.017
- ISSN
- 1226-086X
- Abstract
- Here, we engineered spheroids by using ECM mimicking nano-fragments (NFs) with fibroblasts and investigated their effect on proliferation and cell/ECM interactions. NF incorporation resulted in formation of a stable spheroid, which improved proliferation and viability of cells by assisting oxygen transport confirmed by LOX-1 staining. In addition, hypoxic and apoptotic genes were significantly downregulated in spheroids with PD-NFs. Furthermore, ECM and cell junction proteins were highly expressed. Overall, our findings suggest that incorporation of NFs within spheroids for assembly with various cell types can be an alternative approach for 3D cell culture in many applications.
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