Inducing angiogenesis with the controlled release of nitric oxide from biodegradable and biocompatible copolymeric nanoparticlesopen access
- Authors
- Yang, Chungmo; Hwang, Hae Hyun; Jeong, Soohyun; Seo, Deokwon; Jeong, Yoon; Lee, Dong Yun; Lee, Kangwon
- Issue Date
- Oct-2018
- Publisher
- DOVE MEDICAL PRESS LTD
- Keywords
- mPEG-PLGA nanoparticles; sprouting angiogenesis; low concentration of nitric oxide; liposomal nanoparticles; amphiphilic polymers
- Citation
- INTERNATIONAL JOURNAL OF NANOMEDICINE, v.13, pp.6517 - 6530
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF NANOMEDICINE
- Volume
- 13
- Start Page
- 6517
- End Page
- 6530
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/4667
- DOI
- 10.2147/IJN.S174989
- ISSN
- 1176-9114
- Abstract
- Purpose: Nitric oxide (NO) can be clinically applied at low concentrations to regulate angiogenesis. However, studies using small molecule NO donors (N-diazeniumdiolate, S-nitrosothiol, etc) have yet to meet clinical requirements due to the short half-life and initial burst-release profile of NO donors. In this study, we report the feasibility of methoxy poly(ethylene glycol)-b-poly(lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (NPs) as NO-releasing polymers (NO-NPs) for inducing angiogenesis. Materials and methods: The mPEG-PLGA copolymers were synthesized by typical ring-opening polymerization of lactide, glycolide and mPEG as macroinitiators. Double emulsion methods were used to prepare mPEG-PLGA NPs incorporating hydrophilic NONOate (diethylenetriamine NONOate). Results: This liposomal NP encapsulates hydrophilic diethylenetriamine NONOate (70%+/- 4%) more effectively than other previously reported materials. The application of NO-NPs at different ratios resulted in varying NO-release profiles with no significant cytotoxicity in various cell types: normal cells (fibroblasts, human umbilical vein endothelial cells and epithelial cells) and cancer cells (C6, A549 and MCF-7). The angiogenic potential of NO-NPs was confirmed in vitro by tube formation and ex vivo through an aorta ring assay. Tubular formation increased 189.8% in NO-NP-treated groups compared with that in the control group. Rat aorta exhibited robust sprouting angiogenesis in response to NO-NPs, indicating that NO was produced by polymeric NPs in a sustained manner. Conclusion: These findings provide initial results for an angiogenesis-related drug development platform by a straightforward method with biocompatible polymers.
- Files in This Item
-
- Appears in
Collections - 서울 공과대학 > 서울 생명공학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/4667)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.