Development of fluorescence-conjugated islet-homing peptide using biopanning for targeted optical imaging of pancreatic islet
- Authors
- Kim, Min Jun; Yu, Jeong Heon; Oh, Mi Hwa; Nam, Yoon Sung; Lee, Dong Yun
- Issue Date
- Jan-2017
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- Islet-homing peptide; Pancreatic islet; Phage display; Biopanning; Islet imaging
- Citation
- JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, v.45, pp.404 - 411
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
- Volume
- 45
- Start Page
- 404
- End Page
- 411
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/4839
- DOI
- 10.1016/j.jiec.2016.10.009
- ISSN
- 1226-086X
- Abstract
- Visualization of pancreatic islet can provide important information for diagnosis and monitoring of pancreatic islets. By using phage display technology, here we newly identified four candidate peptide sequences with islet-homing property. In vitro binding assay showed that LSALPRT peptide had stronger affinity with islet cells than other candidates. Then this islet-homing peptide (IHP) was chemically conjugated with TAMRA fluorophore for near infrared (NIR) imaging of islet cells. This TMARA-IHP was strongly bound to islets in vitro, but also to cryo-sectioned slice of pancreas tissue ex vivo. Collectively, it would be a considerable potential as therapeutics or diagnostics in future studies.
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