A New Electrolytic Synthesis Method for Few-Layered MoS2 Nanosheets and Their Robust Biointerfacing with Reduced Antibodies
- Authors
- Kukkar, Manil; Tuteja, Satish K.; Sharma, Amit L.; Kumar, Vinod; Paul, Ashok K.; Kim, Ki-Hyun; Sabherwal, Priyanka; Deep, Akash
- Issue Date
- Jul-2016
- Publisher
- AMER CHEMICAL SOC
- Keywords
- few-layered nanosheets; MoS2; reduced antibody; immunosensor; prostate-specific antigen; FET
- Citation
- ACS APPLIED MATERIALS & INTERFACES, v.8, no.26, pp.16555 - 16563
- Indexed
- SCIE
SCOPUS
- Journal Title
- ACS APPLIED MATERIALS & INTERFACES
- Volume
- 8
- Number
- 26
- Start Page
- 16555
- End Page
- 16563
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/5009
- DOI
- 10.1021/acsami.6b03079
- ISSN
- 1944-8244
- Abstract
- We report an efficient method for the synthesis of few-layered MoS2 nanosheets and demonstrate their application in the label-free detection of the prostate-specific antigen (PSA) cancer marker. As a novel strategy, the electro-dissolution of molybdenum metal sheets in the presence of Na+ and S2- ions led to the formation of Na+ intercalated MoS2. Further exfoliation by ultrasonication yielded the desired formation of few-layered MoS2 nanosheets. After comprehensive characterization, the synthesized MoS2 nanosheets were channeled in a field-effect transistor (FET) microdevice. Chemically reduced anti-PSA antibodies were immobilized on the MoS2 channel above the FET microdevice to construct a specific PSA immunosensor. The antibodies were deliberately reduced to expose the hinge-region disulfide bonds. This approach offered a robust and site-directed immunosensing device through biointerfacing of the sulfhydryl groups (SH) in the reduced antibody with the surface S atoms of MoS2. This device was validated as an effective immunosensor with a low detection limit (10(-5)ng/mL) over a wide linear detection range (10(-5) to 75 ng/mL).
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