Conjugate of graphene quantum dots and glutaminase for the sensing of L-glutamine: Electrochemical vs. fluorescent sensing approaches
- Authors
- Devi, Pooja; Kukkar, Deepak; Kaur, Manpreet; Thakur, Anupma; Kim, Ki-Hyun; Kukkar, Preeti; Kaur, Kamalpreet; Kaur, Harsimran
- Issue Date
- Aug-2021
- Publisher
- Elsevier B.V.
- Keywords
- Cyclic voltammetry; Glutaminase; Glutamine; Graphene quantum dots; Sensing
- Citation
- Inorganic Chemistry Communications, v.130, pp.1 - 7
- Indexed
- SCIE
SCOPUS
- Journal Title
- Inorganic Chemistry Communications
- Volume
- 130
- Start Page
- 1
- End Page
- 7
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/950
- DOI
- 10.1016/j.inoche.2021.108745
- ISSN
- 1387-7003
- Abstract
- This research describes the synthesis of a covalent bioconjugate of graphene quantum dots (GQDs) and glutaminase (10 units·mg−1) for electrochemical and photoluminescent (PL) detection of L-glutamine (Gln). As synthesized GQDs exhibited uniform size distribution, (average diameter ~5–10 nm, and discrete blue emission characteristics (λex = 391 ± 2 nm and λem = 480 ± 2 nm)). Accordingly, the immobilization of glutaminase over the surface of the GQDs was confirmed by the characteristic Fourier-transform infrared (FTIR) spectroscopy peaks of the amide bond at 1,703 and 1,390 cm−1. The conjugate was used to detect Gln using electrochemical and PL approaches on a parallel basis over a concentration range of 100–1,000 µM. Electrochemical analysis of Gln using cyclic voltammetry revealed a limit of detection (LOD) and limit of quantification (LOQ) values of 360.4 µM and 1,100 µM, respectively. In comparison, quantification of Gln based on the PL approach yielded a Stern-Volmer quenching constant, LOD, and LOQ values of 2.2 × 103 M−1, 9.42 µM, and 28.57 µM, respectively. As such, the PL spectroscopy-based analysis was seen to have better sensitivity for Gln quantification than the electrochemical analysis. The PL-based approach is a more recommendable option for Gln quantification with high sensitivity and rapidity.
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