Development of glutaric acid production consortium system with ��-ketoglutaric acid regeneration by glutamate oxidase in Escherichia coli
- Authors
- Yang; S.-Y.; Choi; T.-R.; Jung; H.-R.; Park; Y.-L.; Han; Y.-H.; Song; H.-S.; Gurav; R.; Bhatia; S.K.; Park, Kyoungmoon; K.; Ahn; J.-O.; Yang; Y.-H.
- Issue Date
- 2020
- Publisher
- Elsevier Inc.
- Keywords
- Catalase; Glutamate oxidase; Glutaric acid; Optimization; α-Ketoglutaric acid
- Citation
- Enzyme and Microbial Technology, v.133
- Journal Title
- Enzyme and Microbial Technology
- Volume
- 133
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/12481
- DOI
- 10.1016/j.enzmictec.2019.109446
- ISSN
- 0141-0229
- Abstract
- Glutaric acid is a C5 dicarboxylic acid that can be used as a building block for bioplastics. Although high concentrations of glutaric acid can be produced by fermentation or bioconversion, a large amount of α-ketoglutaric acid (α-KG) is necessary to accept the amine group from 5-aminovaleric acid. To decrease the demand for α-KG, we introduced L-glutamate oxidase (GOX) from Streptomyces mobaraensis in our previous system for cofactor regeneration in combination with a glutaric acid production system from 5-aminovaleric acid. To enhance glutaric acid production, critical factors were optimized such as the expression vector, pH, temperature, and cell ratio. As a result, the demand for α-KG was decreased by more than 6-fold under optimized conditions. Additionally, the effect of catalase was also demonstrated by blocking the degradation of α-KG to succinic acid because of the hydrogen peroxide. Finally, 468.5 mM glutaric acid was produced from 800 mM 5-aminovaleric acid using only 120 mM α-KG. Moreover, this system containing davBA, gabTD-nox, and gox can be applied to produce glutaric acid from L-lysine by reusing α-KG with GOX. This improved cofactor regeneration system has a potential to apply much larger production of glutaric acid. © 2019 Elsevier Inc.
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Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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