Revealing of sugar utilization systems in Halomonas sp. YLGW01 and application for poly(3-hydroxybutyrate) production with low-cost medium and easy recovery
- Authors
- Park, Ye-Lim; Song, Hun-Suk; Choi, Tae-Rim; Lee, Sun Mi; Park, Sol Lee; Lee, Hye Soo; Kim, Hyun-Joong; Bhatia, Shashi Kant; Gurav, Ranjit; Park, Kyungmoon; Yang, Yung-Hun
- Issue Date
- 15-Jan-2021
- Publisher
- ELSEVIER
- Keywords
- Carbon utilization; Halomonas; PHB microbead; Poly(3-hydroxybutyrate); Whole genome sequencing
- Citation
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.167, pp.151 - 159
- Journal Title
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
- Volume
- 167
- Start Page
- 151
- End Page
- 159
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/15630
- DOI
- 10.1016/j.ijbiomac.2020.11.163
- ISSN
- 0141-8130
- Abstract
- Poly(3-hydroxybutyrate) (PHB) is a common polyhydroxyalkanoate (PHA) with potential as an alternative for petroleum-based plastics. Previously, we reported a new strain, Halomonas sp. YLGW01, which hyperproduces PHB with 94% yield using fructose. In this study, we examined the PHB production machinery of Halomonas sp. YLGW01 in more detail by deep-genome sequencing, which revealed a 3,453,067-bp genome with 65.1% guanine-cytosine content and 3054 genes. We found two acetyl-CoA acetyltransferases (Acetoacetyl-CoA thiolase, PhaA), one acetoacetyl-CoA reductase (PhaB), two PHB synthases (PhaC1, PhaC2), PHB depolymerase (PhaZ), and Enoyl-CoA hydratase (PhaJ) in the genome, alongwith two fructose kinases and fructose transporter systems, including the phosphotransferase system (PTS) and ATP-binding transport genes. We then examined the PHB production by Halomonas sp. YLGW01 using high-fructose corn syrup (HFCS) containing fructose, glucose, and sucrose in sea water medium, resulting in 7.95 +/- 0.11 g/L PHB (content, 67.39 +/- 0.34%). PHB was recovered from Halomonas sp. YLGW01 using different detergents; the use of Tween 20 and SDS yielded micro-sized granules with high purity. Overall, these results reveal the distribution of PHB synthetic genes and the sugar utilization system in Halomonas sp. YLGW01 and suggest a possible method for PHB recovery. (C) 2020 Elsevier B.V. All rights reserved.
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