Adipose-Derived Stem Cells and BMP-2 Delivery in Chitosan-Based 3D Constructs to Enhance Bone Regeneration in a Rat Mandibular Defect Model
- Authors
- Fan, Jiabing; Park, Hyejin; Lee, Matthew K.; Bezouglaia, Olga; Fartash, Armita; Kim, Jinku; Aghaloo, Tara; Lee, Min
- Issue Date
- Aug-2014
- Publisher
- MARY ANN LIEBERT, INC
- Citation
- TISSUE ENGINEERING PART A, v.20, no.15-16, pp.2169 - 2179
- Journal Title
- TISSUE ENGINEERING PART A
- Volume
- 20
- Number
- 15-16
- Start Page
- 2169
- End Page
- 2179
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/16642
- DOI
- 10.1089/ten.tea.2013.0523
- ISSN
- 1937-3341
- Abstract
- Reconstructing segmental mandiblular defects remains a challenge in the clinic. Tissue engineering strategies provide an alternative option to resolve this problem. The objective of the present study was to determine the effects of adipose-derived stem cells (ASCs) and bone morphogenetic proteins-2 (BMP-2) in three-dimensional (3D) scaffolds on mandibular repair in a small animal model. Noggin expression levels in ASCs were downregulated by a lentiviral short hairpin RNA strategy to enhance ASC osteogenesis (ASCs(Nog-)). Chitosan (CH) and chondroitin sulfate (CS), natural polysaccharides, were fabricated into 3D porous scaffolds, which were further modified with apatite coatings for enhanced cellular responses and efficient delivery of BMP-2. The efficacy of 3D apatite-coated CH/CS scaffolds supplemented with ASCs(Nog-) and BMP-2 were evaluated in a rat critical-sized mandibular defect model. After 8 weeks postimplantation, the scaffolds treated with ASCs(Nog-) and BMP-2 significantly promoted rat mandibular regeneration as demonstrated by micro-computerized tomography, histology, and immunohistochemistry, compared with the groups treated with ASCs(Nog-) or BMP-2 alone. These results suggest that our combinatorial strategy of ASCs(Nog-) + BMP-2 in 3D apatite microenvironments can significantly promote mandibular regeneration, and these may provide a potential tissue engineering approach to repair large bony defects.
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Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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