Tomentosin Displays Anti-Carcinogenic Effect in Human Osteosarcoma MG-63 Cells via the Induction of Intracellular Reactive Oxygen Species
- Authors
- Lee, Chang Min; Lee, Jongsung; Nam, Myeong Jin; Choi, Youn Soo; Park, See-Hyoung
- Issue Date
- 21-Mar-2019
- Publisher
- MDPI
- Keywords
- osteosarcoma; tomentosin; reactive oxygen species (ROS)
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.20, no.6
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- Volume
- 20
- Number
- 6
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/1832
- DOI
- 10.3390/ijms20061508
- ISSN
- 1661-6596
- Abstract
- Tomentosin is a natural sesquiterpene lactone extracted from various plants and is widely used as a medicine because it exhibits essential therapeutic properties. In this study, we investigated the anti-carcinogenic effects of tomentosin in human osteosarcoma MG-63 cells by performing cell migration/viability/proliferation, apoptosis, and reactive oxygen species (ROS) analysis assays. MG-63 cells were treated with various doses of tomentosin. After treatment with tomentosin, MG-63 cells were analyzed using the MTT assay, colony formation assay, cell counting assay, wound healing assay, Boyden chamber assay, zymography assay, cell cycle analysis, FITC Annexin V apoptosis assay, terminal deoxynucleotidyl transferase dUTP nick end labeling assay, western blot analysis, and ROS detection analysis. Our results indicated that tomentosin decreased cell viability and migration ability in MG-63 cells. Moreover, tomentosin induced apoptosis, cell cycle arrest, DNA damage, and ROS production in MG-63 cells. Furthermore, tomentosin-induced intracellular ROS decreased cell viability and induced apoptosis, cell cycle arrest, and DNA damage in MG-63 cells. Taken together, our results suggested that tomentosin exerted anti-carcinogenic effects in MG-63 cells by induction of intracellular ROS.
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Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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