A simple and cost-effective method to prepare template DNAs for cell-free protein synthesis
- Authors
- Byun, Ju-Young; Park, Kyungmoon; Kim, Dong-Myung
- Issue Date
- Jan-2010
- Publisher
- KOREAN INSTITUTE CHEMICAL ENGINEERS
- Keywords
- Plasmid; Cell-free Protein Synthesis; Scale-up; Extraction
- Citation
- KOREAN JOURNAL OF CHEMICAL ENGINEERING, v.27, no.1, pp.179 - 182
- Journal Title
- KOREAN JOURNAL OF CHEMICAL ENGINEERING
- Volume
- 27
- Number
- 1
- Start Page
- 179
- End Page
- 182
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/20859
- DOI
- 10.1007/s11814-009-0294-4
- ISSN
- 0256-1115
- Abstract
- The scale-up of cell-free protein synthesis reactions involves the preparation of large amounts of template DNA. While ion-exchange column chromatography methods have commonly been used to obtain purified plasmid DNA for cell-free protein synthesis reactions, these methods are costly and difficult to expand to a large scale. In this work, we report that the routine isopropyl alcohol (IPA) precipitation method can be used to prepare cell-free-expressible DNA when the co-precipitated proteins are removed. Compared to column-purification procedures, the IPA-precipitation offers obvious advantages with respect to the cost and scaling-up of template preparation, and we believe that our finding will contribute to making cell-free protein synthesis system more practical for the rapid production of preparative amounts of recombinant proteins.
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Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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