Effect of Glutaraldehyde Treatment on Stability of Permeabilized Ochrobactrum anthropi SY509 in Nitrate Removal
- Authors
- Park, Young Tae; Park, Jae Yeon; Park, Kyungmoon; Choi, Suk Soon; Yoo, Young Je
- Issue Date
- Nov-2008
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- Denitrification; permeabilization; glutaraldehyde; stability; immobilization
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.18, no.11, pp.1803 - 1808
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 18
- Number
- 11
- Start Page
- 1803
- End Page
- 1808
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/22644
- DOI
- 10.4014/jmb.0800.094
- ISSN
- 1017-7825
- Abstract
- For practical application, the stability of permeabilized Ochrobactrum anthropi SY509 needs to be increased, as its half-life of enzymatic denitrification is only 90 days. As the cells become viable after permeabilization treatment, this can cause decreased activity in a long-term operation and induce breakage of the immobilization matrix. However, the organic solvent concentration causing zero cell viability was 50%, which is too high for industrial application. Thus, whole-cell immobilization using glutaraldehyde was performed, and 0.1% (v/v) glutaraldehyde was determined as the optimum concentration to maintain activity and increase the half-life. It was also found that 0.1% (v/v) glutaraldehyde reacted with 41.9% of the total amine residues on the surface of the cells during the treatment. As a result, the half-life of the permeabilized cells was increased from 90 to 210 days by glutaraldehyde treatment after permeabilization, and no cell viability was detected.
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Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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