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Recent advances in DNA sequencing by End-Labeled Free-Solution Electrophoresis (ELFSE)

Authors
Won, Jong-In
Issue Date
May-2006
Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
Keywords
DNA sequencing; capillary electrophoresis (CE); microfluidic device; ELFSE; drag-tag; microchannel electrophoresis
Citation
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.11, no.3, pp.179 - 186
Journal Title
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
Volume
11
Number
3
Start Page
179
End Page
186
URI
https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/24561
DOI
10.1007/BF02932028
ISSN
1226-8372
Abstract
End-Labeled Free-Solution Electrophoresis (ELFSE) is a new technique that is a promising bioconjugate method for DNA sequencing (or separation) and genotyping by both capillary and microfluidic device electrophoresis. Because ELFSE enables high-resolution electrophoretic separation in aqueous buffer alone (i.e., without a polymer matrix), it eliminates the need to load viscous polymer networks into electrophoresis microchannels. To achieve microchannel DNA separations with high performance, ELFSE requires monodisperse perturbing entities (i.e., drag-tags), which create a large amount of frictional drag when pulled behind DNA during free-solution electrophoresis, and which have other properties suitable for microchannel electrophoresis. In this article, the theoretical concepts of ELFSE and the required characteristics of the drag-tag molecules for the ultimate performance of ELFSE are reviewed. Additionally, the merits and limitations of current drag-tags are also discussed in the context of recent experimental data of ELFSE separation (or sequencing).
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