Ratiometric fluorescent detection of lead ions in aquatic environment and living cells using a fluorescent peptide-based probe
- Authors
- Mehta, P.K.; Jeon, J.; Ryu, K.; Park, S.-H.; Lee, K.-H.
- Issue Date
- 5-Apr-2022
- Publisher
- Elsevier B.V.
- Keywords
- Fluorescent; Lead; Pb(II); Probe; Sensor
- Citation
- Journal of Hazardous Materials, v.427
- Journal Title
- Journal of Hazardous Materials
- Volume
- 427
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/25307
- DOI
- 10.1016/j.jhazmat.2021.128161
- ISSN
- 0304-3894
- Abstract
- Ratiometric fluorescent detection using dual emission bands is highly necessary to quantify Pb(II) in aquatic environment and live cells. We synthesized a ratiometric fluorescent peptidyl probe (1) by conjugation of a peptide receptor for Pb(II) with an excimer-forming benzothiazolylcyanovinylene fluorophore. The peptidyl probe dissolved well in aqueous solution and displayed an emission band at 538 nm (λex = 460 nm). Upon addition of Pb(II) (0–20 μM), the emission maximum shifted from 538 nm to 575 nm and the emission intensity ratio (I575 /I538) increased significantly from 0.40 to 2.26. 1 exhibited a selective ratiometric response to Pb(II) over other metal ions. 1 with a low detection limit (1.2 ppb) of Pb(II) detected nanomolar concentrations (0–500 nM) of Pb(II) ions in groundwater and tap water. The cell-permeable probe detected intracellular Pb(II) by ratiometric fluorescent images. The binding mode study using NMR, IR and CD spectroscopy, and TEM revealed that the probe formed a 1:1 complex with Pb(II) and then formed red-emissive nanoparticles and fibrils. The probe exhibited desirable detection properties such as ratiometric detection, high solubility in water, visible light excitation, high selectivity and sensitivity for Pb(II), cell-permeability, and rapid response (< 6 min). © 2022 Elsevier B.V.
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Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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