Fed-batch production of D-ribose from sugar mixtures by transketolase-deficient Bacillus subtilis SPK1
- Authors
- Park, YC; Kim, SG; Park, K; Lee, KH; Seo, JH
- Issue Date
- Dec-2004
- Publisher
- SPRINGER
- Citation
- APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.66, no.3, pp.297 - 302
- Journal Title
- APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 66
- Number
- 3
- Start Page
- 297
- End Page
- 302
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/25719
- DOI
- 10.1007/s00253-004-1678-3
- ISSN
- 0175-7598
- Abstract
- D-Ribose, a five-carbon sugar, is used as a key intermediate for the production of various biomaterials, such as riboflavin and inosine monophosphate. A high D-ribose-producing Bacillus subtilis SPK1 strain was constructed by the chemical mutation of the transketolase-deficient strain, B. subtilis JY1. Batch fermentation of B. subtilis SPK1 with 20 g l(-1) xylose and 20 g l(-1) glucose resulted in 4.78 g l(-1) dry cell mass, 23.0 g l(-1) D-ribose concentration, and 0.72 g l(-1) h(-1) productivity, corresponding to a 1.5- to 1.7-fold increase when compared with values for the parental strain. A late-exponential phase was chosen as the best point for switching to a fed-batch process. Optimized fed-batch fermentation of B. subtilis SPK1, feeding a mixture of 200 g l(-1) xylose and 50 g l(-1) glucose after the late-exponential phase reduced the residual xylose and glucose concentrations to less than 7.0 g l(-1) and gave the best results of 46.6 g l(-1) D-ribose concentration and 0.88 g l(-1) h(-1) productivity which were 2.0- and 1.2-fold higher than the corresponding values in a simple batch fermentation.
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