Generation of a novel antibody against BxPrx, a diagnostic marker of pine wilt disease
- Authors
- Kim, S.-H.; Yun, H.-Y.; Jeong, H.-J.
- Issue Date
- 1-May-2023
- Publisher
- Springer Science and Business Media B.V.
- Keywords
- Bursaphelenchus xylophilus; Immunoassay; Monoclonal antibody; Phage display; Pine wilt disease
- Citation
- Molecular Biology Reports, v.50, no.5, pp 4715 - 4721
- Pages
- 7
- Journal Title
- Molecular Biology Reports
- Volume
- 50
- Number
- 5
- Start Page
- 4715
- End Page
- 4721
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/30952
- DOI
- 10.1007/s11033-023-08342-3
- ISSN
- 0301-4851
1573-4978
- Abstract
- Background: Bursaphelenchus xylophilus is a pathogenic nematode that causes pine wilt disease (PWD). To prevent the rapid spread of this pathogen, developing a method for rapid and accurate detection of B. xylophilus is required. Methods and results: In this study, we produced a B. xylophilus peroxiredoxin (BxPrx), which is a protein that is overexpressed in B. xylophilus. Using recombinant BxPrx as an antigen, we generated and selected a novel antibody that binds to BxPrx via phage display and biopanning. We subcloned the anti-BxPrx single-chain variable fragment-encoding phagemid DNA to mammalian expression vector. We transfected the plasmid into mammalian cells and produced a highly sensitive recombinant antibody that enabled nanogram order detection of BxPrx. Conclusion: The sequence of anti-BxPrx antibody as well as the rapid immunoassay system described here can be applied for rapid and accurate diagnosis of PWD. © 2023, The Author(s), under exclusive licence to Springer Nature B.V.
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Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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