Enhanced production of glutaric acid by biocatalyst-recycled bioconversion process integrated with in situ product recovery by adsorption
- Authors
- Cha, Haeng-Geun; Kim, Hee Taek; Park, See-Hyoung; Kong, Youjung; Yi, Byongson; Wang, Jimin; Song, Eunchae; Joo, Jeong Chan; Yang, Yung-Hun; Ahn, Jung-Oh; Park, Kyungmoon
- Issue Date
- Dec-2023
- Publisher
- Elsevier Inc.
- Keywords
- Glutaric acid; Glutaric acid adsorption; in situ product recovery; Product inhibition; Whole-cell bioconversion
- Citation
- Enzyme and Microbial Technology, v.171
- Journal Title
- Enzyme and Microbial Technology
- Volume
- 171
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/31699
- DOI
- 10.1016/j.enzmictec.2023.110307
- ISSN
- 0141-0229
1879-0909
- Abstract
- Product inhibition caused by organic acids is a serious issue in establishing economical biochemical production systems. Herein, for enhanced production of glutaric acid by overcoming product inhibition triggered by glutaric acid, a whole-cell bioconversion system equipped with biocatalyst recycling process and in situ product recovery by adsorption was developed successfully. From the whole-cell bioconversion reaction, we found that both dissociated and undissociated forms of glutaric acid acted as an inhibitor in the whole-cell bioconversion reaction, wherein bioconversion was hindered beyond 200 mM glutaric acid regardless of reaction pH. Therefore, as the promising solution for the inhibition issue by glutaric acid, the biocatalyst-recycled bioconversion process integrated with in situ product recovery by adsorption was introduced in the whole-cell bioconversion. As a result, 592 mM glutaric acid was produced from 1000 mM 5-aminovaleric acid with 59.2% conversion. We believe that our system will be a promising candidate for economically producing organic acids with high titer. © 2023 Elsevier Inc.
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Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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