Development of fluorescence-linked immunosorbent assay for rapid detection of <i>Staphylococcus aureus</i>
- Authors
- Kim, Joo-Kyung; Yun, Hyun-Young; Kim, Jae-Seok; Kim, Wooseong; Lee, Chang-Soo; Kim, Byung-Gee; Jeong, Hee-Jin
- Issue Date
- Dec-2024
- Publisher
- SPRINGER
- Keywords
- Staphylococcus aureus; Fluorescent antibody; Immunoassay; FLISA; ELISA
- Citation
- APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.108, no.1, pp 1 - 10
- Pages
- 10
- Journal Title
- APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 108
- Number
- 1
- Start Page
- 1
- End Page
- 10
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/32608
- DOI
- 10.1007/s00253-023-12836-2
- ISSN
- 0175-7598
1432-0614
- Abstract
- Staphylococcus aureus is a major pathogen that causes infections and life-threatening diseases. Although antibiotics, such as methicillin, have been used, methicillin-resistant S. aureus (MRSA) causes high morbidity and mortality rates, and conventional detection methods are difficult to be used because of time-consuming process. To control the spread of S. aureus, a development of a rapid and simple detection method is required. In this study, we generated a fluorescent anti-S. aureus antibody, and established a novel fluorescence-linked immunosorbent assay (FLISA)-based S. aureus detection method. The method showed high sensitivity and low limit of detection toward MRSA detection. The assay time for FLISA was 5 h, which was faster than that of conventional enzyme-linked immunosorbent assay (ELISA) or rapid ELISA. Moreover, the FLISA-based detection method was applied to diagnose clinically isolated MRSA samples that required only 5.3 h of preincubation. The FLISA method developed in this study can be widely applied as a useful tool for convenient S. aureus detection.
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