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Soluble expression of recombinant coagulation factor IX protein using Escherichia coliopen access
- Authors
- Park, Byoung-Hee; Yun, Hanool; Jeong, Hee-Jin
- Issue Date
- Jul-2024
- Publisher
- Elsevier B.V.
- Keywords
- Escherichia coli; Factor IX; Hemophilia; Protein expression; Recombinant protein
- Citation
- Biochemistry and Biophysics Reports, v.38
- Journal Title
- Biochemistry and Biophysics Reports
- Volume
- 38
- ISSN
- 2405-5808
- Abstract
- Hemophilia B is a congenital bleeding disorder caused by factor IX (FIX) deficiency. Generation of recombinant FIX (rFIX) is required for detecting a Hemophilia B indicator, anti-FIX antibody. In this study, we described a method for producing recombinant FIX (rFIX) using Escherichia coli. We constructed a FIX-expressing plasmid without a fusion tag protein-encoding gene and produced rFIX as a soluble form within five days. Dose-dependent curve was obtained from ELISA using anti-FIX antibody, indicating that the rFIX can be used as an antigen to detect anti-FIX antibody with high affinity and sensitivity. © 2024 The Authors
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Related Researcher
- Jeong, Hee-Jin
- Science & Technology (Biological and Chemical Engineering)