Optimization of Direct Lysine Decarboxylase Biotransformation for Cadaverine Production with Whole-Cell Biocatalysts at High Lysine Concentration
- Authors
- Kim, Hyun Joong; Kim, Yong Hyun; Shin, Ji-Hyun; Bhatia, Shashi Kant; Sathiyanarayanan, Ganesan; Seo, Hyung-Min; Choi, Kwon Young; Yang, Yung-Hun; Park, Kyungmoon
- Issue Date
- Jul-2015
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- Biotransformation; whole-cell biocatalyst; lysine decarboxylase; cadaverine; high-concentration reaction; Escherichia coli
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.25, no.7, pp.1108 - 1113
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 25
- Number
- 7
- Start Page
- 1108
- End Page
- 1113
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/9673
- DOI
- 10.4014/jmb.1412.12052
- ISSN
- 1017-7825
- Abstract
- Cadaverine (1,5-diaminopentane) is an important industrial chemical with a wide range of applications. Although there have been many efforts to produce cadaverine through fermentation, there are not many reports of the direct cadaverine production from lysine using biotransformation. Whole-cell reactions were examined using a recombinant Escherichia coli strain overexpressing the E. coli MG1655 cadA gene, and various parameters were investigated for the whole-cell bioconversion of lysine to cadaverine. A high concentration of lysine resulted in the synthesis of pyridoxa1-5'-phosphate (PLP) and it was found to be a critical control factor for the biotransformation of lysine to cadaverine. When 0.025 mM PLP and 1.75 M lysine in 500 mM sodium acetate buffer (pH6) were used, consumption of 91% lysine and conversion of about 80% lysine to cadaverine were successfully achieved.
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Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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