Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor
- Authors
- Kim, Dae Hoon; Oh, Hong Gi; Park, Woo Hwan; Jeon, Dong Cheol; Lim, Ki Moo; Kim, Hyung Jin; Jang, Byoung Kuk; Song, Kwang Soup
- Issue Date
- Nov-2018
- Publisher
- MDPI
- Keywords
- hepatocellular carcinoma; alpha-fetoprotein; biosensor; graphene; field-effect transistor
- Citation
- SENSORS, v.18, no.11
- Journal Title
- SENSORS
- Volume
- 18
- Number
- 11
- URI
- https://scholarworks.bwise.kr/kumoh/handle/2020.sw.kumoh/19116
- DOI
- 10.3390/s18114032
- ISSN
- 1424-8220
- Abstract
- The detection of alpha-fetoprotein (AFP) in plasma is important in the diagnosis of hepatocellular carcinoma (HCC) in humans. We developed a biosensor to detect AFP in HCC patient plasma and in a phosphate buffer saline (PBS) solution using a graphene field-effect transistor (G-FET). The G-FET was functionalized with 1-pyrenebutyric acid N-hydroxysuccinimide ester (PBASE) for immobilization of an anti-AFP antibody. AFP was detected by assessing the shift in the voltage of the Dirac point (Delta V-Dirac) after binding of AFP to the anti-AFP-immobilized G-FET channel surface. This anti-AFP-immobilized G-FET biosensor was able to detect AFP at a concentration of 0.1 ng mL(-1) in PBS, and the detection sensitivity was 16.91 mV. In HCC patient plasma, the biosensor was able to detect AFP at a concentration of 12.9 ng mL(-1), with a detection sensitivity of 5.68 mV. The sensitivity (Delta V-Dirac) depended on the concentration of AFP in either PBS or HCC patient plasma. These data suggest that G-FET biosensors could have practical applications in diagnostics.
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Collections - Department of Medical IT Convergence Engineering > 1. Journal Articles
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