Selective Homocysteine Assay with Cucurbit[7]uril by pH RegulationSelective Homocysteine Assay with Cucurbit[7]uril by pH Regulation
- Other Titles
- Selective Homocysteine Assay with Cucurbit[7]uril by pH Regulation
- Authors
- Bae Won-Bin; Kim Hee-Joon; 지광환
- Issue Date
- Apr-2022
- Publisher
- 한국미생물·생명공학회
- Keywords
- Homocysteine; cysteine; cucurbit[7]uril; homocystine; pH effect; supramolecular complexation
- Citation
- Journal of Microbiology and Biotechnology, v.32, no.4, pp.514 - 521
- Journal Title
- Journal of Microbiology and Biotechnology
- Volume
- 32
- Number
- 4
- Start Page
- 514
- End Page
- 521
- URI
- https://scholarworks.bwise.kr/kumoh/handle/2020.sw.kumoh/21104
- DOI
- 10.4014/jmb.2201.01016
- ISSN
- 1017-7825
- Abstract
- We report the effect of pH on the supramolecular complexation of two biothiols, viz., homocysteine (Hcy) and cysteine (Cys), with cucurbit[7]uril (CB[7]). Under basic pH conditions, Cys did not complex with CB[7], whereas Hcy efficiently complexed with CB[7], as confirmed by 1H NMR spectroscopy and Ellman’s reagent (5,5’-dithio-bis(2-nitrobenzoic acid), DTNB) assay. 1H NMR and Raman spectroscopic studies revealed that, in the absence of CB[7], Hcy auto-oxidized slowly (~36 h) to homocystine (HSSH) under basic pH conditions. However, the rate of Hcy oxidation increased by up to 150 fold in the presence of CB[7], as suggested by the DTNB assay. Thus, supramolecular complexation under basic pH conditions led to the formation of a HSSH-CB[7] complex, and not Hcy- CB[7]. The results indicate that Hcy is rapidly oxidized to HSSH under the catalysis of CB[7], which acts as a reaction chamber, in basic pH conditions. Our studies suggest that Hcy concentration, a risk factor for cardiovascular disease, can be selectively and more easily quantified by supramolecular complexation with CB [7].
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Collections - Department of Applied Chemistry > 1. Journal Articles
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