Multifunctional chitosan-bimetallic nanocarrier deliver 5-fluorouracil for enhanced treatment of pancreatic and triple-negative breast cancer
- Authors
- Saravanakumar, Kandasamy; Sathiyaseelan, Anbazhagan; Manivasagan, Panchanathan; Zhang, Xin; Jeong, Myeong Seon; Jang, Eue-Soon; Wang, Myeong-Hyeon
- Issue Date
- Feb-2024
- Publisher
- ELSEVIER
- Keywords
- Au/Pd nanoparticles; 5-Fluorouracil; Chitosan; Aptamer; Cytotoxicity
- Citation
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.259
- Journal Title
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
- Volume
- 259
- URI
- https://scholarworks.bwise.kr/kumoh/handle/2020.sw.kumoh/26617
- DOI
- 10.1016/j.ijbiomac.2023.129165
- ISSN
- 0141-8130
1879-0003
- Abstract
- This work aimed to prepare multifunctional aptamer-conjugated, photothermally responsive 5-fluorouracil (5fu)-loaded chitosan-bimetallic (Au/Pd) nanoparticles (APT-CS-5fu-Au/Pd NPs) for improved cytotoxicity in two cancer cell lines (PANC-1 and MDA-MD 231). The CS-5fu-Au/Pd NPs were polydispersed with a size of 34.43 +/- 1.59 nm. FTIR analysis indicated the presence of CS, 5fu in CS-5fu-Au/Pd NPs. The 2 theta degrees in CS-5fu-Au/Pd NPs accounted for CS and Au/Pd. Additionally, AGE revealed the conjugation of APT in CS-5fu-Au/Pd NPs. The APT-CS-5fu-Au/Pd NPs (180 mu g/mL) with NIR treatment increased the temperature to >50 degrees C. The optimized 5fu input was 0.075 % in CS-5fu-Au/Pd NPs, exhibiting a hydrodynamic size of 112.96 +/- 17.23 nm, DEE of 64.2 +/- 3.77 %, and DLE of 11.1 +/- 0.65 %. A higher level of 5fu release (69.8 +/- 2.78 %) was observed under pH 5.4 at 74 h. In conclusion, NIR-APT-CS-5fu-Au/Pd NPs did not cause toxicity to RBC and Egg CAM, but increased cytotoxicity in MDA-MB 231 and PANC-1 cells by triggering oxidative stress-mediated cell death.
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