Bioassay-guided isolation and identification of anti-platelet-active compounds from the root of Ashitaba (Angelica keiskei Koidz.)
- Authors
- Son, Dong Ju; Park, Ye Oak; Yu, Chengguang; Lee, Sung Eun; Park, Young Hyun
- Issue Date
- 2014
- Publisher
- Taylor & Francis
- Keywords
- Angelica keiskei Koidaz; Ashitaba; anti-platelet; 4-hydroxyderricin; xanthoangelol
- Citation
- Natural Product Research, v.28, no.24, pp 2312 - 2316
- Pages
- 5
- Journal Title
- Natural Product Research
- Volume
- 28
- Number
- 24
- Start Page
- 2312
- End Page
- 2316
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/13021
- DOI
- 10.1080/14786419.2014.931389
- ISSN
- 1478-6419
1478-6427
- Abstract
- Platelet aggregation is fundamental to a wide range of physiological and pathological processes, including the induction of thrombosis and arteriosclerosis. Anti-platelet activity of a crude methanol extract and solvent fractions of Ashitaba roots (Angelica keiskei Koidz.) was evaluated using a turbidimetric method using washed rabbit platelets. We identified the anti-platelet activities of two chalcones, 4-hydroxyderricin and xanthoangelol, isolated from the ethyl acetate-soluble fraction of Ashitaba roots by using a bioassay-guided isolation method. 4-Hydroxyderricin and xanthoangelol effectively inhibited platelet aggregation induced by collagen (IC50 of 41.9 and 35.9 mu M, respectively), platelet-activating factor (IC50 of 46.1 and 42.3 mu M, respectively) and phorbol 12-myristate 13-acetate (IC50 of 16.5 and 45.9 mu M, respectively). These compounds did not inhibit thrombin-induced platelet aggregation (IC50 of > 80 mu M). The results suggest that the chalcones 4-hydroxyderricin and xanthoangelol may be potent anti-thrombotic components of A. keiskei Koidz.
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