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A Highly Facile and Specific Assay for Cancer-Causing Isocitrate Dehydrogenase Mutant Using C-13(4)-Labeled alpha-Ketoglutarate and Heteronuclear NMR
- Authors
- Wen, He; Yun, Taeho; Xu, Wen Jun; Choi, Seung Hong; Kim, Hyeonjin; Park, Chul-Kee; Lee, Se-Hoon; Park, Sung-woo; Lee, Sang Kook; Park, Sunghyouk
- Issue Date
- 17-Dec-2013
- Publisher
- American Chemical Society
- Citation
- Analytical Chemistry, v.85, no.24, pp 11987 - 11992
- Pages
- 6
- Journal Title
- Analytical Chemistry
- Volume
- 85
- Number
- 24
- Start Page
- 11987
- End Page
- 11992
- ISSN
- 0003-2700
1520-6882
- Abstract
- Isocitrate dehydrogenase mutations with neomorphic activity of converting a-ketoglutarate to 2-hydroxyglutarate have been found in many types of cancers. We report an NMR-based assay specific for the mutant using C-13(4)-labeled alpha-ketoglutarate. It can be done in a complex mixture without extraction, give time-dependent absolute quantitation, and be applied to enzyme inhibition studies. Its merits over conventional assays should facilitate inhibitor developments for a new class of target-oriented anticancer agents.
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Related Researcher
- Park, Sung woo
- College of Medicine (Department of Internal Medicine)