Molecular and immunohistochemical characterization of granulin gene encoded in Pieris rapae granulovirus genome
- Authors
- Oh, Seunghan; Kim, Dong Hyun; Patnaik, Bharat Bhusan; Jo, Yong Hun; Noh, Mi Young; Lee, Hyo Jeong; Lee, Kwang Ho; Yoon, Kwang Ho; Kim, Wan-Jong; Noh, Ju Young; Jeong, Heon Cheon; Lee, Yong Seok; Zhang, Chuan-Xi; Kim, Iksoo; Han, Yeon Soo
- Issue Date
- May-2013
- Publisher
- Academic Press
- Keywords
- Pieris rapae granulovirus; PiraGV-Korea; granulin; gene expression; immunohistochemistry
- Citation
- Journal of Invertebrate Pathology, v.113, no.1, pp 7 - 17
- Pages
- 11
- Journal Title
- Journal of Invertebrate Pathology
- Volume
- 113
- Number
- 1
- Start Page
- 7
- End Page
- 17
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/13739
- DOI
- 10.1016/j.jip.2012.12.003
- ISSN
- 0022-2011
1096-0805
- Abstract
- Pieris rapae granulovirus (PiraGV) is highly pathogenic to the cabbage butterfly (P. rapae), an important pest of cultivated cabbages and mustard crops. It therefore holds significant promise towards exploitation as a potent bio-control agent in the field controlling the pest population. Whole-genome elucidation of the Korean isolate of the granulovirus (PiraGV-K), reported the presence of a granulin gene corresponding to ORF 1 in its genome. Comprehensive studies towards functional characterization of the gene, established that it is composed of 744 nucleotides and encodes a peptide of 247 amino acid residues. It possessed significant homology with AoGV and ClanGV with 87% identity at amino acid level. Multiple alignment data suggests that the C-terminus region of the gene had three different conserved regions. Time-course studies conducted in PiraGV-K infected P. rapae larvae revealed a significant upsurge of the transcript (134-fold) at 4 days post infection followed by a significant decline at the most advanced stages of infection. Anti-PiraGV-K granulin antibody was produced and western blot conducted with the infected larvae further confirmed the induction pattern with a protein of 30 kDa. Immunofluorescent staining showed a granulin-specific signal in fat body and integument of the infected larvae. Granulin-specific signals were noticed 2 days post infection with the eventual systemic spread of infection to the associated tracheal matrix witnessed at 4 days post infection. Immunogold labeling and electron microscopic studies further proved the cytopathological effects as the presence of numerous membrane-bound vesicles with nucleocapsids and abruption of intercellular junctions in fat body and hypertrophied cells in the integument. (c) 2012 Elsevier Inc. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Natural Sciences > Department of Biology > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/13739)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.