A Methanol Extract of Adansonia digitata L. Leaves Inhibits Pro-Inflammatory iNOS Possibly via the Inhibition of NF-kappa B Activation
- Authors
- Ayele, Yihunie; Kim, Jung-Ah; Park, Eunhee; Kim, Ye-Jung; Retta, Negussie; Dessie, Gulelat; Rhee, Sang-Ki; Koh, Kwangoh; Nam, Kung-Woo; Kim, Hee Seon
- Issue Date
- 31-Mar-2013
- Publisher
- 한국응용약물학회
- Keywords
- Adansonia digitata; Antioxidant; Anti-inflammatory; NF-kappa B; I kappa B; iNOS
- Citation
- Biomolecules & Therapeutics, v.21, no.2, pp 146 - 152
- Pages
- 7
- Journal Title
- Biomolecules & Therapeutics
- Volume
- 21
- Number
- 2
- Start Page
- 146
- End Page
- 152
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/13822
- DOI
- 10.4062/biomolther.2012.098
- ISSN
- 1976-9148
2005-4483
- Abstract
- This study examined the total polyphenol content of eight wild edible plants from Ethiopia and their effect on NO production in Raw264.7 cells. Owing to its relatively high polyphenol concentration and inhibition of NO production, the methanol extract of Adansonia digitata L. leaf (MEAD) was subjected to detailed evaluation of its antioxidant and anti-inflammatory effects. Antioxidant effects were assessed by measuring free-radical-scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and oxygen-radical-absorbance capacity (ORAC) assays, while anti-inflammatory effects were assessed by measuring inducible nitric oxide synthase (iNOS) expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. In the ORAC assay, MEAD was 10.2 times more potent than vitamin C at eliminating peroxyl radicals. In DPPH assay, MEAD also showed a strong ROS scavenging effect. MEAD significantly inhibited iNOS activity (IC50=28.6 mu g/ml) of LPS-stimulated Raw264.7 cells. We also investigated the relationship between iNOS expression and nuclear factor kappa B (NF-kappa B) activation. MEAD inhibited I kappa B alpha degradation and NF-kappa B translocation from the cytosol to the nucleus in LPS-induced RAW264.7 cells without significant cytotoxic effects, as confirmed by MTT assay. These results suggest that MEAD inhibits anti-inflammatory iNOS expression, which might be related to the elimination of peroxyl radicals and thus the inhibition of I kappa B alpha-mediated NF-kappa B signal transduction.
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Collections - College of Natural Sciences > Department of Food Science and Nutrition > 1. Journal Articles
- College of Natural Sciences > Department of Biology > 1. Journal Articles
- College of Natural Sciences > Department of Chemistry > 1. Journal Articles
- College of Medical Sciences > Department of Pharmaceutical Engineering > 1. Journal Articles
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