Mast Cell Stabilizing Effect of (-)-Elema-1,3,11(13)-trien-12-ol and Thujopsene from Thujopsis dolabrata Is Mediated by Down-Regulation of Interleukin-4 Secretion in Antigen-Induced RBL-2H3 Cells
- Authors
- Kim, Chul-Hee; Lee, Taehun; Oh, Ikhoon; Nam, Kung-Woo; Kim, Kyeong Ho; Oh, Ki-Bong; Shin, Jongheon; Mar, Woongchon
- Issue Date
- Mar-2013
- Publisher
- Pharmaceutical Society of Japan
- Keywords
- Thujopsis dolabrata; RBL-2H3 cell; mast cell degranulation; interleukin-4; beta-hexosaminidase
- Citation
- Biological and Pharmaceutical Bulletin, v.36, no.3, pp 339 - 345
- Pages
- 7
- Journal Title
- Biological and Pharmaceutical Bulletin
- Volume
- 36
- Number
- 3
- Start Page
- 339
- End Page
- 345
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/13895
- DOI
- 10.1248/bpb.b12-00375
- ISSN
- 0918-6158
1347-5215
- Abstract
- Several isolated compounds from the wood part of Thujopsis dolabrata were evaluated for their inhibitory effects against antigen-induced mast cell degranulation and interleukin-4 (IL-4) secretion, as well as IL-4 mRNA and protein expression in immunoglobulin E (IgE)-sensitized RBL-2H3 cells. Among the five isolated compounds, (-)-elema-1,3,11(13)-trien-12-ol (1) and thujopsene (2) exhibited the potent inhibitory activity against mast cell degranulation measured by beta-hexosaminidase release with IC50 values of 27.4 mu m and 25.1 mu m, respectively. These compounds also inhibited the release of IL-4 (IC50 values of 7.0, 6.7 mu m, respectively), IL-4 mRNA expression (IC50 values of 16.5, 7.2 mu m, respectively) and IL-4 protein expression (IC50 values of 17.0, 9.6 mu m, respectively) in antigen-induced IgE-sensitized RBL-2H3 cells. These results suggested that (-)-elema-1,3,11(13)-trien-12-ol (1) and thujopsene (2) effectively inhibits mast cell degranulation as well as IL-4 production, suggesting that these compounds from Thujopsis dolabrata can be used as candidates for IgE-mediated allergic disorders.
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