Antioxidant Activity and Inhibition of Lipid Peroxidation in Germinating Seeds of Transgenic Soybean Expressing OsHGGT
- Authors
- Kim, Yul Ho; Lee, Yu Young; Kim, Yong Ho; Choi, Man Soo; Jeong, Kwang Ho; Lee, Seuk Ki; Seo, Min Jung; Yun, Hong Tai; Lee, Choon Ki; Kim, Wook Han; Lee, Sang Chul; Park, Soon Ki; Park, Hyang Mi
- Issue Date
- 26-Jan-2011
- Publisher
- American Chemical Society
- Keywords
- Tocotrienols; tocopherols; seed germination; rice homogentisate geranylgeranyl transferase (OsHGGT); antioxidant activity; lipid peroxidation; soybean
- Citation
- Journal of Agricultural and Food Chemistry, v.59, no.2, pp 584 - 591
- Pages
- 8
- Journal Title
- Journal of Agricultural and Food Chemistry
- Volume
- 59
- Number
- 2
- Start Page
- 584
- End Page
- 591
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/16753
- DOI
- 10.1021/jf104012z
- ISSN
- 0021-8561
- Abstract
- Tocochromanols are potent lipid-soluble antioxidants and essential nutrients for human health. Genetic engineering techniques were used to develop soybeans with enhanced vitamin E levels, including tocotrienols, which are not found in soybean. The gene encoding rice homogentisate geranylgeranyl transferase (HGGT) was overexpressed in soybeans using seed-specific and constitutive promoters. The association between abundance of vitamin E isomers and antioxidant activity was investigated during seed germination. With the exception of beta-tocotrienol, all vitamin E isomers were detected in germinating seeds expressing OsHGGT. The antioxidant properties of germinating seed extracts were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radicals and lipid peroxidation (TBARS). Compared with intact wild-type seeds, transgenic seeds showed increases in radical scavenging of 5.4-17 and 23.2-35.3% in the DPPH and ABTS assays, respectively. Furthermore, the lipid peroxidation levels were 2.0-4.5-fold lower in germinating seeds from transgenic lines than in wild-type seeds. Therefore, it appears that the antioxidant potential of transgenic oil-producing plants such as soybean, sunflower, and corn may be enhanced by overexpressing OsHGGT during seed germination.
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Collections - College of Medical Sciences > Department of Medical Biotechnology > 1. Journal Articles
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