A Clinical Trial to Evaluate the Efficacy of alpha-Viniferin in Staphylococcus aureus - Specific Decolonization without Depleting the Normal Microbiota of Naresopen access
- Authors
- Rahim, Md Abdur; Seo, Hoonhee; Kim, Sukyung; Jeong, Yoon Kyoung; Tajdozian, Hanieh; Kim, Mijung; Lee, Saebim; Song, Ho-Yeon
- Issue Date
- Mar-2021
- Publisher
- Polskie Towarzystwo Mikrobiologow/Polish Society of Microbiologists
- Keywords
- alpha-viniferin; clinical trial; MRSA; PCR; 16S rRNA amplicon sequencing analysis
- Citation
- Polish Journal of Microbiology, v.70, no.1, pp 117 - 130
- Pages
- 14
- Journal Title
- Polish Journal of Microbiology
- Volume
- 70
- Number
- 1
- Start Page
- 117
- End Page
- 130
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/19098
- DOI
- 10.33073/pjm-2021-011
- ISSN
- 1733-1331
2544-4646
- Abstract
- Staphylococcus aureus is currently a significant multidrug-resistant bacterium, causing severe healthcare-associated and community-acquired infections worldwide. The current antibiotic regimen against this pathogen is becoming ineffective due to resistance, in addition, they disrupt the normal microbiota. It highlights the urgent need for a pathogen-specific drug with high antibacterial efficacy against S. aureus. alpha-Viniferin, a bioactive phytochemical compound, has been reported to have excellent anti-Staphylococcus efficacy as a topical agent. However, so far, there were no clinical trials that have been conducted to elucidate its efficacy. The present study aimed to investigate the antibacterial efficacy of alpha-viniferin against S. aureus in a ten-day clinical trial. Based on the results, alpha-viniferin showed 50% minimum inhibitory concentrations (MIC50 values) of 7.8 mu g/ml in culture broth medium. alpha-Viniferin was administered in the nares three times a day for ten days using a sterile cotton swab stick. Nasal swab specimens were collected before (0 days) and after finishing the trial (10th day), and then analyzed. In the culture and RT-PCR-based analysis, S. ureus was reduced significantly: 0.01. In addition, 16S ribosomal RNA-based amplicon sequencing analysis showed that S. aureus reduced from 51.03% to 23.99% at the genus level. RNA-seq analysis was also done to gain insights into molecular mechanisms of alpha-viniferin against S. aureus, which revealed that some gene groups were reduced in 5-fold FC cutoff at two times MIC conditions. The study results demonstrate alpha-viniferin as a potential S. aureus-specific drug candidate.
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