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Micro- and nano-tomography analysis of mouse soleus muscle using radiation

Authors
Jang, Sang-HunLee, JiwonLee, Onseok
Issue Date
Nov-2021
Publisher
John Wiley & Sons Inc.
Keywords
actin; myofibril; myosin; X‐ ray microtomography; X‐ ray nanotomography
Citation
Microscopy Research and Technique, v.84, no.11, pp 2685 - 2693
Pages
9
Journal Title
Microscopy Research and Technique
Volume
84
Number
11
Start Page
2685
End Page
2693
URI
https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/19315
DOI
10.1002/jemt.23819
ISSN
1059-910X
1097-0029
Abstract
In this study, we analyze radiation images of muscle structure of mice soleus muscles using radiation source-based microtomography and nanotomography. Soleus muscle samples were collected for analysis from 8-week-old male Institute of Cancer Research mice. First, phase-contrast X-ray microtomography was employed in these experiments. Then to obtain images with excellent contrast, imaging was performed using monochromatic light with excellent transmission power. To analyze additional muscle structures in higher magnification images than these images, nanotomography was performed, which facilitated obtaining high-magnification and high-resolution images. Muscle tissue microstructures were confirmed through three-dimensional images obtained from phase-contrast X-ray microtomography. Thus, the muscle tissue's overall shape at microscopic level can be captured. Additionally, a single muscle fiber was examined using hard X-ray nano-imaging, through which we could observe the alignment of countless myofibrils, that is, actin and myosin filaments in the muscle fibers. Thus, the methodology adopted here proved to be advantageous in analyzing the muscle tissue's overall structure with microtomography and in observing the myofibrils in detail using nanotomography.
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