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Factors of Endoscopic Ultrasound-Guided Tissue Acquisition for Successful Next-Generation Sequencing in Pancreatic Ductal Adenocarcinoma

Authors
Park, Jae KeunLee, Ji HyeonNoh, Dong HyoPark, Joo KyungLee, Kyu TaekLee, Jong KyunLee, Kwang HyuckJang, Kee-TaekCho, Juhee
Issue Date
May-2020
Publisher
거트앤리버 발행위원회
Keywords
Next-generation sequencing; Endoscopic ultrasound-guided tissue acquisition; Endoscopic ultrasound guided fine-needle aspiration; Endoscopic ultrasound guided fine-needle biopsy; Pancreatic ductal adenocarcinoma
Citation
Gut and Liver, v.14, no.3, pp 387 - 394
Pages
8
Journal Title
Gut and Liver
Volume
14
Number
3
Start Page
387
End Page
394
URI
https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/19531
DOI
10.5009/gnl19011
ISSN
1976-2283
2005-1212
Abstract
Background/Aims: Recent advances in understanding the genetics of pancreatic ductal adenocarcinoma (PDAC) have led to the potential for a personalized approach. Several studies have described the feasibility of generating genetic profiles of PDAC with next-generation sequencing (NGS) of samples obtained through endoscopic ultrasound-guided tissue acquisition (EUS-TA). The aim of this study was to find the best EUS-TA approach for successful NGS of PDAC. Methods: We attempted to perform NGS with tissues from 190 patients with histologically proven PDAC by endoscopic ultrasound-guided fine-needle aspiration and endoscopic ultrasound-guided fine-needle biopsy at Samsung Medical Center between November 2011 and February 2015. The medical records of these patients were retrospectively reviewed for parameters including tumor factors (size, location, and T stage), EUS-TA factors (needle gauge [G], needle type, and number of needle passes) and histologic factors (cellularity and blood contamination). The sample used for NGS was part of the EUS-TA specimen that underwent cytological and histological analysis. Results: NGS could be successfully performed in 109 patients (57.4%). In the univariate analysis, a large needle G (p=0.003) and tumor located in the body/tail (p=0.005) were associated with successful NGS. The multivariate logistic regression analysis revealed that the needle G was an independent factor of successful NGS (odds ratio, 2.19; 95% confidence interval, 1.08 to 4.47: p=0.031). Conclusions: The needle G is an independent factor associated with successful NGS. This finding may suggest that the quantity of cells obtained from EUS-TA specimens is important for successful NGS.
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