Factors of Endoscopic Ultrasound-Guided Tissue Acquisition for Successful Next-Generation Sequencing in Pancreatic Ductal Adenocarcinoma
- Authors
- Park, Jae Keun; Lee, Ji Hyeon; Noh, Dong Hyo; Park, Joo Kyung; Lee, Kyu Taek; Lee, Jong Kyun; Lee, Kwang Hyuck; Jang, Kee-Taek; Cho, Juhee
- Issue Date
- May-2020
- Publisher
- 거트앤리버 발행위원회
- Keywords
- Next-generation sequencing; Endoscopic ultrasound-guided tissue acquisition; Endoscopic ultrasound guided fine-needle aspiration; Endoscopic ultrasound guided fine-needle biopsy; Pancreatic ductal adenocarcinoma
- Citation
- Gut and Liver, v.14, no.3, pp 387 - 394
- Pages
- 8
- Journal Title
- Gut and Liver
- Volume
- 14
- Number
- 3
- Start Page
- 387
- End Page
- 394
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/19531
- DOI
- 10.5009/gnl19011
- ISSN
- 1976-2283
2005-1212
- Abstract
- Background/Aims: Recent advances in understanding the genetics of pancreatic ductal adenocarcinoma (PDAC) have led to the potential for a personalized approach. Several studies have described the feasibility of generating genetic profiles of PDAC with next-generation sequencing (NGS) of samples obtained through endoscopic ultrasound-guided tissue acquisition (EUS-TA). The aim of this study was to find the best EUS-TA approach for successful NGS of PDAC. Methods: We attempted to perform NGS with tissues from 190 patients with histologically proven PDAC by endoscopic ultrasound-guided fine-needle aspiration and endoscopic ultrasound-guided fine-needle biopsy at Samsung Medical Center between November 2011 and February 2015. The medical records of these patients were retrospectively reviewed for parameters including tumor factors (size, location, and T stage), EUS-TA factors (needle gauge [G], needle type, and number of needle passes) and histologic factors (cellularity and blood contamination). The sample used for NGS was part of the EUS-TA specimen that underwent cytological and histological analysis. Results: NGS could be successfully performed in 109 patients (57.4%). In the univariate analysis, a large needle G (p=0.003) and tumor located in the body/tail (p=0.005) were associated with successful NGS. The multivariate logistic regression analysis revealed that the needle G was an independent factor of successful NGS (odds ratio, 2.19; 95% confidence interval, 1.08 to 4.47: p=0.031). Conclusions: The needle G is an independent factor associated with successful NGS. This finding may suggest that the quantity of cells obtained from EUS-TA specimens is important for successful NGS.
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