Micro-injection Molded Droplet Generation System for Digital PCR Application
- Authors
- Jo, Daae; Kim, So Young; Kang, Hyeon Woo; Pyo, Sung Han; Jeong, Nam Kyu; Bae, Nam Ho; Lee, Seok Jae; Kim, Yong Tae; Lee, Kyoung G.
- Issue Date
- Dec-2022
- Publisher
- 한국바이오칩학회
- Keywords
- Digital nucleic acid assay; Microdroplet; Micropattern; Digital PCR; Metal mold
- Citation
- BioChip Journal, v.16, no.4, pp 433 - 440
- Pages
- 8
- Journal Title
- BioChip Journal
- Volume
- 16
- Number
- 4
- Start Page
- 433
- End Page
- 440
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/21641
- DOI
- 10.1007/s13206-022-00079-8
- ISSN
- 1976-0280
2092-7843
- Abstract
- Sensitive, effective, and quantitative analysis of infectious pathogens is an important task for the prevention of human health threats. Herein, we present an advanced approach to producing gene-encapsulated microdroplets for quantitative analysis using a micropatterned metal mold and injection molding technique with an automatically operated system. An injection molded microdroplet generation device was successfully fabricated with a minimum channel width of 30 mu m and optimized to produce 100 mu m diameter droplets. The optimized microchannel design and flow rate also enable the production of stable numbers of microdroplets (similar to 16,000 droplets). To verify the applicability of our device and system to droplet-based digital PCR analysis, Escherichia coli (E. coli) O157:H7 was selected as a model bacterial pathogen, and the stx2 gene was amplified in the microdroplets. The generated microdroplets exhibit both chemical and mechanical stability, and our results are similar to those obtained by a commercially available method. Accordingly, the usefulness of the microdroplet generative device and system is confirmed as a simple, fast, and reliable tool for the quantitative molecular analysis of infectious diseases.
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Collections - College of Natural Sciences > Department of Food Science and Nutrition > 1. Journal Articles
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