Next-generation diagnostic test for dengue virus detection using an ultrafast plasmonic colorimetric RT-PCR strategyopen access
- Authors
- Jiang, Kunlun; Lee, Jung-Hoon; Fung, To Sing; Wu, Jingrui; Liu, Congnuan; Mi, Hua; Rajapakse, R. P. V. Jayanthe; Balasuriya, Udeni B. R.; Peng, Yung-Kang; Go, Yun Young
- Issue Date
- Sep-2023
- Publisher
- ELSEVIER
- Keywords
- Biosensor; Plasmonic photothermal PCR; Photothermal effect; Colorimetric; Dengue diagnosis
- Citation
- ANALYTICA CHIMICA ACTA, v.1274
- Journal Title
- ANALYTICA CHIMICA ACTA
- Volume
- 1274
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/25467
- DOI
- 10.1016/j.aca.2023.341565
- ISSN
- 0003-2670
1873-4324
- Abstract
- The current global COVID-19 pandemic once again highlighted the urgent need for a simple, cost-effective, and sensitive diagnostic platform that can be rapidly developed for distribution and easy access in resource-limited areas. Here, we present a simple and low-cost plasmonic photothermal (PPT)-reverse transcription-colorimetric polymerase chain reaction (RTcPCR) for molecular diagnosis of dengue virus (DENV) infection. The assay can be completed within 54 min with an estimated detection limit of 1.6 copies/mu L of viral nucleic acid. The analytical sensitivity and specificity of PPT-RTcPCR were comparable to that of the reference RT-qPCR assay. Moreover, the clinical performance of PPT-RTcPCR was evaluated and validated using 158 plasma samples collected from patients suspected of dengue infection. The results showed a diagnostic agreement of 97.5% compared to the reference RT-qPCR and demonstrated a clinical sensitivity and specificity of 97.0% and 100%, respectively. The simplicity and reliability of our PPT-RTcPCR strategy suggest it can provide a foundation for developing a field -deployable diagnostic assay for dengue and other infectious diseases.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.