A novel decellularized skeletal muscle-derived ECM scaffolding system for in situ muscle regeneration
- Authors
- Lee, Hyeongjin; Ju, Young Min; Kim, Ickhee; Elsangeedy, Ebrahim; Lee, Joon Ho; Yoo, James J.; Atala, Anthony; Lee, Sang Jin
- Issue Date
- 15-Jan-2020
- Publisher
- Academic Press
- Keywords
- Decellularization; Skeletal muscle; Extracellular matrix; Insulin-like growth factor 1; Tissue engineering; In situ tissue regeneration
- Citation
- Methods, v.171, pp 77 - 85
- Pages
- 9
- Journal Title
- Methods
- Volume
- 171
- Start Page
- 77
- End Page
- 85
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/3175
- DOI
- 10.1016/j.ymeth.2019.06.027
- ISSN
- 1046-2023
1095-9130
- Abstract
- The cell-based tissue engineering strategies have gained attention in restoring normal tissue function after skeletal muscle injuries; however, these approaches require a donor tissue biopsy and extensive cell expansion process prior to implantation. In order to avoid this limitation, we developed a novel cell-free muscle-specific scaffolding system that consisted of a skeletal muscle-derived decellularized extracellular matrix (dECM) and a myogenic factor, insulin growth factor-1 (IGF-1). Rheological, morphological, and biological properties of this muscle-specific scaffold (IGF-1/dECM) as well as collagen and dECM scaffolds were examined. The cell viability in all scaffolds had over 90% at 1, 3, and 7 days in culture. The cell proliferation in the IGF-1/dECM was significantly increased when compared with other groups. More importantly, the IGF-1/dECM strongly supported the myogenic differentiation in the scaffold as confirmed by myosin heavy chain (MHC) immunofluorescence. We also investigated the feasibility in a rabbit tibialis anterior (TA) muscle defect model. The IGF1/dECM had a significantly greater number of myofibers when compared to both collagen and dECM groups at 1 and 2 months after implantation. We demonstrated that this novel muscle-specific scaffolding system could effectively promote the muscle tissue regeneration in situ.
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Collections - College of Medicine > Department of Anesthesiology > 1. Journal Articles
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