MicroRNA-27 inhibits adipogenic differentiation in orbital fibroblasts from patients with Graves' orbitopathyopen access
- Authors
- Jang, Sun Young; Chae, Min Kyung; Lee, Joon H.; Lee, Eun Jig; Yoon, Jin Sook
- Issue Date
- 15-Aug-2019
- Publisher
- Public Library of Science
- Keywords
- Graves' ophthalmopathy
- Citation
- PLoS ONE, v.14, no.8
- Journal Title
- PLoS ONE
- Volume
- 14
- Number
- 8
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/4311
- DOI
- 10.1371/journal.pone.0221077
- ISSN
- 1932-6203
- Abstract
- Background To investigate the role of microRNA (miR)-27a and miR-27b in adipogenesis in an in vitro model of Graves' orbitopathy (GO). Methods Orbital fat tissues were harvested from GO and non-GO participants for primary orbital fibro-blast cultures. The expression levels of miR-27a and miR-27b between GO and non-GO orbital fat tissues were compared. During adipogenesis of GO orbital fibroblasts, the expression levels of miR-27a and miR-27b were determined, and the effects of mimics of miR-27a and miR-27b transfection on adipogenesis of GO orbital fibroblast were investigated. Results Real time-polymerase chain reaction showed significantly more decreases in miR-27a and miR-27b levels in orbital fat tissues in GO participants than in non-GO participants (p < 0.05). The expression of both miR-27a and miR-27b was highest in orbital fibroblasts at day 0 and declined gradually after the induction of adipogenic differentiation. The expression levels of PPAR gamma, CCAAT/enhancer binding protein (C/EBP)alpha and C/EBP beta were decreased and Oil Red O-stained lipid droplets were lower in GO orbital fibroblasts transfected with miR-27a and miR-27b mimics than in negative controls. Conclusions Our results indicated that miR-27a and miR-27b inhibited adipogenesis in orbital fibroblasts from GO patients. Further studies are required to examine the potential of miR-27a and miR-27b as targets for therapeutic strategies.
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Collections - College of Medicine > Department of Ophthalmology > 1. Journal Articles
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