The role of Wnt/beta-catenin signaling in the restoration of induced pluripotent stem cell-derived retinal pigment epithelium after laser photocoagulation
- Authors
- Cho, In Hwan; Park, Seong Jun; Lee, Si Hyung; Nah, Seung Kwan; Park, Ha Yan; Yang, Jin Young; Madrakhimov, Sanjar Batirovich; Lyu, Jungmook; Park, Tae Kwann
- Issue Date
- Apr-2019
- Publisher
- Springer Verlag
- Keywords
- Wnt/beta-catenin signaling pathway; Induced pluripotent stem cell; Retinal pigment epithelium; Laser photocoagulation
- Citation
- Lasers in Medical Science, v.34, no.3, pp 571 - 581
- Pages
- 11
- Journal Title
- Lasers in Medical Science
- Volume
- 34
- Number
- 3
- Start Page
- 571
- End Page
- 581
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/4628
- DOI
- 10.1007/s10103-018-2631-5
- ISSN
- 0268-8921
1435-604X
- Abstract
- To investigate the role of Wnt/-catenin signaling pathway in the restoration of induced pluripotent stem cell-derived retinal pigment epithelium (hiPSC-RPE) after laser photocoagulation. After differentiation of RPE cells from hiPSCs, laser photocoagulation was performed. Activation of Wnt/-catenin signaling at days 1 and 5 after laser photocoagulation was evaluated by expression of -catenin. Cell proliferation and alteration in cell-to-cell contact at day 5 after laser photocoagulation with or without Dickkopf-1 (Dkk-1) treatment were studied using ethynyl-2-deoxyuridine (EdU) assay and zonula occludens-1 (ZO-1) expression analysis, respectively. The mRNA levels of Wnt genes at day 5 after laser photocoagulation were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). Activation of Wnt/-catenin signaling at days 1 and 5 after laser photocoagulation was confirmed by -catenin accumulation in the cytoplasm and nucleus of hiPSC-RPE. Many EdU-positive cells also expressed -catenin, and the number of EdU-positive cells was decreased at day 5 after laser photocoagulation after Dkk-1 treatment, indicating that Wnt/-catenin signaling mediated hiPSC-RPE proliferation. ZO-1 expression was not decreased with Dkk-1 treatment at day 5 after laser photocoagulation, indicating that Wnt/-catenin signaling mediated hiPSC-RPE restoration. At day 5, after laser photocoagulation, mRNA levels of Wnt2b, Wnt3, Wnt5a, Wnt7a, and Wnt10b were increased. Wnt/-catenin signaling has a crucial role in restoration of hiPSC-RPE proliferation after laser photocoagulation. Manipulation of Wnt/-catenin signaling while elucidating the underlying mechanisms of RPE restoration might have a therapeutic potential in retinal degenerative diseases.
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Collections - College of Medicine > Department of Ophthalmology > 1. Journal Articles
- College of Medicine > Department of Ophthalmology > 1. Journal Articles
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