In-depth study of lin-28 suggests selectively conserved let-7 independent mechanism in Drosophila
- Authors
- Lee, Mihye; Thanh My Thi Nguyen; Kim, Kiyoung
- Issue Date
- 1-Mar-2019
- Publisher
- Elsevier BV
- Keywords
- Lin-28; let-7; RNA binding protein; Embryonic development; Drosophila
- Citation
- Gene, v.687, pp 64 - 72
- Pages
- 9
- Journal Title
- Gene
- Volume
- 687
- Start Page
- 64
- End Page
- 72
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/4664
- DOI
- 10.1016/j.gene.2018.11.017
- ISSN
- 0378-1119
1879-0038
- Abstract
- Lin-28 is a conserved RNA-binding protein that is involved in a wide range of developmental processes and pathogenesis. At the molecular level, Lin-28 blocks the maturation of let-7 and regulates translation of certain mRNA targets. In Drosophila, Lin-28 is reported to play a role in oogenesis, muscle formation, and the symmetric division of adult intestinal stem cells. In this study, we characterized Drosophila Lin-28 through a detailed examination of its temporal and spatial expression. Lin-28 is specifically expressed in embryonic nervous and cardiac systems. However, loss or gain of lin-28 function does not cause any abnormality during embryonic development. Instead, the ubiquitous overexpression of Lin-28 leads to lethality from late larval stage to pupal stage, and eye-specific overexpression causes severe cell loss. The ectopic expression of human Lin28A has the same effect as Drosophila Lin-28, indicating functional conservation in Lin-28 orthologs. We also examined the effect of Lin-28 on let-7 biogenesis through the mutant and overexpression analysis. Lin-28 does not block the production of let-7 in Drosophila, which suggests the let-7 independent pathway as a molecular mechanism of Lin-28.
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- Appears in
Collections - College of Medical Sciences > Department of Medical Biotechnology > 1. Journal Articles
- Graduate School > Department of Integrated Biomedical Science > 1. Journal Articles
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